CCT3 Antibody

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Western Blot: CCT3 Antibody [NBP1-51949] - Analysis of CCT3 in Mouse Testis lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CCT3 Antibody Summary

Immunogen
Peptide with sequence C-GHKKKGDDQSRQGG corresponding to C-Terminus (near) according to NP_001008883.1, NP_001008800.1.
Epitope
C-GHKKKGDDQSRQGG
Specificity
This antibody is expected to recognize all reported isoforms (NP_005989.3; NP_001008883.1; NP_001008800.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCT3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 65 kDa band observed in mouse and rat testis lysates (calculated MW of 60.5 kDa band according to human NP_005989.3 and 60.6 kDa band according to mouse NP_033966.1 and rat NP_954522.1).
Publications
Read Publication using NBP1-51949.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Porcine, Bovine, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCT3 Antibody

  • Cctg
  • chaperonin containing TCP1, subunit 3 (gamma)
  • hTRiC5
  • TCP1 (t-complex-1) ring complex, polypeptide 5
  • TRIC5gamma subunit

Background

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCT3 Antibody (NBP1-51949)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCT3
Entrez
Uniprot