CCT3 Antibody

Western Blot: CCT3 Antibody [NBP1-51949] - Analysis of CCT3 in Mouse Testis lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Hu, Po, Bv, Ca
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CCT3 Antibody Summary

• Immunogen: Peptide with sequence C-GHKKKGDDQSRQGG corresponding to C-Terminus (near) according to NP_001008883.1, NP_001008800.1.
• Epitope: C-GHKKKGDDQSRQGG
• Specificity: This antibody is expected to recognize all reported isoforms (NP_005989.3; NP_001008883.1; NP_001008800.1).
• Predicted Species: Human (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 65 kDa band observed in mouse and rat testis lysates (calculated MW of 60.5 kDa band according to human NP_005989.3 and 60.6 kDa band according to mouse NP_033966.1 and rat NP_954522.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CCT3 Antibody

• Cctg
• chaperonin containing TCP1, subunit 3 (gamma)
• hTRiC5
• TCP1 (t-complex-1) ring complex, polypeptide 5
• TRIC5gamma subunit

Background

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCT3 Antibody (NBP1-51949)(1)

Publication using NBP1-51949

• Barbe L, Lundberg E, Oksvold P et al. Toward a confocal subcellular atlas of the human proteome. Mol Cell Proteomics 2008-03-01 [PMID: 18029348]

Bioinformatics

• Gene Symbol: CCT3
• Entrez:
  • 7203(Human)
  • 12462(Mouse)
  • 295230(Rat)
• Uniprot:
  • NP_005989.3(Human)