Cbx2 Antibody (880833)

Cbx2 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated with 0.2 µM retinoic acid for 4 days (panels A and C) or undifferentiated (panels B and D), using Mouse Anti-Human Cbx2 Monoclonal Antibody (Catalog # MAB8098) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panels; Catalog # NL007) and counterstained with DAPI (blue, lower panels). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 880833
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cbx2 Antibody (880833) Summary

• Immunogen: E. coli-derived recombinant human Cbx2
  Lys131-His217
  Accession # Q14781
• Specificity: Detects human Cbx2 in ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CBX2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cbx2 Antibody (880833)

• Cbx2
• CDCA6
• cell division cycle associated 6
• chromobox homolog 2 (Drosophila Pc class)
• chromobox homolog 2 (Pc class homolog, Drosophila)
• chromobox homolog 2
• chromobox protein homolog 2
• M33
• MGC10561
• modifier 3
• Pc class homolog
• SRXY5

Background

Chromobox homolog 2 (Cbx2), also known as CDCA6 and M33, is an approximately 56 kDa protein component of the nuclear polycomb repressive complex 1 (PRC1). PRC1 regulates chromatin organization and gene transcription. Cbx2 interacts with methylated histone H3, and this is regulated by phosphorylation of Ser42 within the Cbx2 chromodomain. Cbx2 is required for male sex determination by activating expression of the Sry gene. It is also important for germ cell viability and meiotic chromosome synapsis. Within amino acids 131-217, human Cbx2 shares 94% and 92% aa sequence identity with mouse and rat Cbx2, respectively. Alternative splicing generates an additional isoform that is substituted and truncated following Lys96.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBX2
• Uniprot:
  • Q14781()