Recombinant Human CBFB His Protein

SDS-Page: Recombinant Human CBFB Protein [NBP1-45317] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human CBFB His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-182 of Human CBFB
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPRVVPDQRS KFENEEFFRK LSRECEIKYT GFRDRPHEER QARFQNACRD GRSEIAFVAT GTNLSLQFFP ASWQGEQRQT PSREYVDLER EAGKVYLKAP MILNGVCVIW KGWIDLQRLD GMGCLEFDEE RAQQEDALAQ QAFEEARRRT REFEDRDRSH REEMEVRVSQ LLAVTGKKTT RP

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CBFB
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 23.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM MES buffer (pH 6.0), 0.1 mM PMSF, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human CBFB His Protein

• CBFB
• CBF-beta
• core-binding factor subunit beta
• core-binding factor, beta subunit
• PEA2-beta
• PEBP2B
• PEBP2-beta
• polyomavirus enhancer binding protein 2, beta subunit
• Polyomavirus enhancer-binding protein 2 beta subunit
• SL3/AKV core-binding factor beta subunit
• SL3-3 enhancer factor 1 beta subunit
• SL3-3 enhancer factor 1 subunit beta
• SL3-3

Background

CBFB, also known as PEBP2B, is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). This protein is a non-DNA binding regulatory subunit and it allosterically enhances DNA binding by alpha subunit. Recombinant human CBFB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CBFB Recombinant Protein (NBP1-45317)(1)

Publication using NBP1-45317

• Hsu CW, Conrad JW, Sowers ML et al. A combinatorial system to examine the enzymatic repair of multiply damaged DNA substrates Nucleic acids research 2022-07-22 [PMID: 35776119]

Bioinformatics

• Gene Symbol: CBFB