CBFB Antibody [Alexa Fluor® 350]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

CBFB Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human CBFB
  Pro2-Glu165
  Accession # Q13951
• Specificity: Detects human and mouse CBFB in Western blots and recombinant human CBFB in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human RUNX-1, -2, and -3 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CBFB Antibody [Alexa Fluor® 350]

• CBFB
• CBF-beta
• core-binding factor subunit beta
• core-binding factor, beta subunit
• PEA2-beta
• PEBP2B
• PEBP2-beta
• polyomavirus enhancer binding protein 2, beta subunit
• Polyomavirus enhancer-binding protein 2 beta subunit
• SL3/AKV core-binding factor beta subunit
• SL3-3 enhancer factor 1 beta subunit
• SL3-3 enhancer factor 1 subunit beta
• SL3-3

Background

CBFB (Core binding Factor beta; also PEA2 beta and PEBP2 beta ) is a widely-expressed 21-24 kDa member of the CBFB family of proteins. It forms dimeric transcriptional complexes with multiple molecules, including CBFB, RUNX1 and RUNX2. Although it does not bind DNA, it potentiates the binding of its partners to DNA. Human CBFB is 182 amino acids (aa) in length. It contains multiple alpha -helices and beta -strands. CBFB has at least two splice variants. One is approximately 23 kDa in size and possesses a 22 aa substitution for aa 166-182. A second is 16 kDa in size and contains a 22 aa substitution for aa 134-182. And a third shows a potential deletion of aa 56-94 coupled to the above 22 substitution for aa 166-182. CBFB is known to form a 68-70 kDa fusion protein with smooth muscle myosin heavy chain in AML. The CBFB contribution to the N-terminus of this fusion protein usually involves aa 1-165. Over aa 1-165, human CBFB shares 98% aa sequence identity with mouse CBFB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.