Casein Kinase 1 delta Antibody

Western Blot: Casein Kinase 1 delta Antibody [NB100-2919] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. Antibody at 0.2 ug/mL.

Immunocytochemistry/Immunofluorescence: Casein Kinase 1 delta Antibody [NB100-2919] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Casein Kinase 1 delta Antibody Summary

• Immunogen: Peptide with sequence C-DREERLRHSRNPATR corresponding to internal region according to NP_620693.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_001884.2 and NP_620693.1)
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSNK1D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/mL
• Control:

  Casein Kinase 1 delta Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Casein Kinase 1 delta Antibody

• Casein Kinase 1 delta
• casein kinase 1, delta
• casein kinase I isoform delta
• CKId
• CKI-delta
• CSNK1D
• EC 2.7.11
• EC 2.7.11.1
• HCKID

Background

CKI delta is a member of the casein kinase 1 (CK1) serine/threonine kinase family of proteins. In mammals, there are seven distinct genes that represent the CK1 family: alpha, beta, gamma-1, gamma-2, gamma-3, delta, and epsilon. CKI delta phosphorylates a number of proteins some of which include the circadian proteins period 1 and period 2. CKI delta is also involved in the Wnt signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Casein Kinase 1 delta Antibody (NB100-2919)(2)

Publications using NB100-2919

• Dibyadeep Datta, Isabella Perone, Denethi Wijegunawardana, Feng Liang, Yury M. Morozov, Jon Arellano, Alvaro Duque, Zhongcong Xie, Christopher H. van Dyck, Mary Kate P. Joyce, Amy F. T. Arnsten Nanoscale imaging of pT217‐tau in aged rhesus macaque entorhinal and dorsolateral prefrontal cortex: Evidence of interneuronal trafficking and early‐stage neurodegeneration Alzheimer's & Dementia 2024-03-06 [PMID: 38445818]
• Xu Y, Padiath QS, Shapiro RE et al. Functional consequences of a CKIdelta mutation causing familial advanced sleep phase syndrome. Nature 2005-03-31 [PMID: 15800623]

Bioinformatics

• Gene Symbol: CSNK1D
• Entrez:
  • 1453(Human)
  • 104318(Mouse)
  • 64462(Rat)
• Uniprot:
  • NP_001884.2(Human)