CARD10 Antibody [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 594

CARD10 Antibody [Alexa Fluor™ Plus 594] Summary

• Immunogen: CARD10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CARD10. The immunogen is located within the first 50 amino acids of CARD10. Amino Acid Squence: MPGRAEAGEAEEEAGA
• Specificity: CARD10 antibody is human specific. At least three isoforms of CARD10 are known to exist; this antibody will only detect isoform 1. CARD10 antibody is predicted not to cross-react with other CARMA proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CARD10
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse: (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CARD10 Antibody [Alexa Fluor™ Plus 594]

• CARD-containing MAGUK 3 protein
• CARD-containing MAGUK protein 3
• Carma 3
• CARMA3BIMP1Bcl10 binding protein and activator of NFKB
• caspase recruitment domain family, member 10
• caspase recruitment domain-containing protein 10
• MGC142219

Background

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. CARDs induce nuclear factor kappa-B (NFKB; MIM 164011) activity through the IKK (e.g., IKBKB; MIM 603258) complex. CARD9 (MIM 607212), CARD10, CARD11 (MIM 607210), and CARD14 (MIM 607211) interact with BCL10 (MIM 603517) and are involved in NFKB signaling complexes. Except for CARD9, these CARD proteins are members of the membrane-associated guanylate kinase (MAGUK) family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CARD10