Carboxypeptidase E/CPE Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 647

Carboxypeptidase E/CPE Antibody [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human Carboxypeptidase E/CPE in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human CPM is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxypeptidase E/CPE Antibody [Alexa Fluor™ Plus 647]

• Carboxypeptidase E
• Carboxypeptidase H
• CPE
• CPH
• CPH-1
• EC 3.4.17
• EC 3.4.17.10
• Enkephalin convertase
• Prohormone-processing carboxypeptidase

Background

Encoded by the CPE gene and also known as Carboxypeptidase H, CPE is a single chain peptidase with an optimal pH range between 5.0‑6.0. It is a zinc metallocarboxypeptidase that removes basic amino acids from the C-terminus of peptides (1). Like other metallocarboxypeptidases, its activity is stimulated by millimolar concentrations of Co²⁺. Its activity is regulated by pH-induced aggregation above pH 6.0. Its major function seems to process numerous peptide hormones and neurotransmitters. In addition to its proteolytic function, it also plays a role as a sorting receptor (2), which may be attributed to the sorting of this protein into the secretory pathway. The C-terminal domain of CPE causes the peripheral association of CPE with membranes below neutral pH, resulting in the association of this protein into membranes (3). CPE knockout mice live but become obese due to impaired glucose clearance and insulin resistance (4).

1. Fricker, L.D. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 840.
2. Cool, D.R. et al. (1997) Cell 88:73.
3. Zhang, C-F. et al.  (2003) Biochem. J. 369:453.
4. Cawley, N.X. et al. (2004) Endocrinology 145:5807.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.