Carboxypeptidase A2/CPA2 Antibody (384014)

• Reactivity: Hu
• Applications: WB, IP, B/N
• Clone: 384014
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Carboxypeptidase A2/CPA2 Antibody (384014) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A2/CPA2
  Tyr17-His417
  Accession # P48052
• Specificity: Detects human Carboxypeptidase A2/CPA2 in direct ELISAs and Western blots. In Western blots, recognizes the pro form but not the activated enzyme.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CPA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Neutralization 1.5 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxypeptidase A2/CPA2 Antibody (384014)

• carboxypeptidase A2 (pancreatic)
• Carboxypeptidase A2
• CPA2
• EC 3.4.17
• EC 3.4.17.15

Background

Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417).

1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPA2
• Uniprot:
  • P48052()