NHA2/SLC9B2/NHEDC2 Antibody (896151) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human NHA2/SLC9B2 and eGFP was stained with and either (A) Mouse Anti-Human NHA2/SLC9B2 Monoclonal Antibody (Catalog # MAB8404) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 896151
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NHA2/SLC9B2/NHEDC2 Antibody (896151) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human NHA2/SLC9B2
  Met1-Val537
  Accession # NP_849155
• Specificity: Detects HEK293 cells transfected with human NHA2/SLC9B2 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC9B2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NHA2/SLC9B2/NHEDC2 Antibody (896151) [Unconjugated]

• FLJ23984
• Mitochondrial Na(+)/H(+) exchanger NHA2
• mitochondrial sodium/hydrogen exchanger NHA2
• Na(+)/H(+) exchanger-like domain-containing protein 2
• Na+/H+ exchanger domain containing 2
• NHA2
• NHA2NHE10
• NHE domain-containing protein 2
• NHE10
• NHEDC2
• SLC9B2
• Sodium/hydrogen exchanger-like domain-containing protein 2

Background

NHA2/SLC9B2 (Solute Carrier family 9, subfamily B, member 2) is a member of the solute carrier family of multi-pass membrane proteins. Sodium hydrogen antiporters, such as SLC9B2 (NHA2), convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux. SLC9B2 contributes to organellar volume homeostasis and is required for osteoclast differentiation and bone resorption activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC9B2
• Uniprot:
  • NP_849155()