Carbohydrate Sulfotransferase 4/CHST4 Antibody [Unconjugated]

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5547) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbohydrate Sulfotransferase 4/CHST4 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Carbohydrate Sulfotransferase 4/CHST4
  Arg30-Gly388
• Specificity: Detects mouse Carbohydrate Sulfotransferase 4/CHST4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human (rh) CHST4 is observed and less than 5% cross‑reactivity with recombinant mouse CHST5 and rhCHST6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CHST4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Neutralization 60 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 4/CHST4 Antibody [Unconjugated]

• carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
• Carbohydrate Sulfotransferase 4
• CHST4
• EC 2.8.2
• EC 2.8.2.-
• EC 2.8.2.17
• EC 2.8.2.21
• galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3
• Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3
• GlcNAc6ST2
• GlcNAc6ST-2
• gn6st-2
• GST3
• GST-3
• HECGLCNAC6ST
• HEC-GlcNAc6ST
• HEC-GLCNAC-6-ST
• High endothelial cells N-acetylglucosamine 6-O-sulfotransferase
• L-selectin ligand sulfotransferase
• LSST
• LSSTcarbohydrate sulfotransferase 4
• N-acetylglucosamine 6-O-sulfotransferase 2

Background

The mouse CHST family is comprised of 13 enzymes. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of the recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). Human CHST4, also known as high endothelial cell N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L‑selectin ligand sulfotransferase (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L‑selectin ligands (4). It has a catalytic preference for core 2-branched mucin-type O‑glycans, but also has activity toward core 3 type of O‑glycans (5). Mouse CHST4 shares 72% amino acid sequence identity with the human ortholog.

1. deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278: 40282.
2. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
3. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
4. Bistrup, A. et al. (1999) J. Cell Biol. 145:899.
5. Uchimura, K. et al. (2002) J. Biol. Chem. 277: 3979.
6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
7. MacRae, I.J. et al. (2000) Biochemistry. 39: 1613.
8. Wu, Z.L. et al. (2002) Faseb J. 16:539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHST4