Carbohydrate Sulfotransferase 4/CHST4 Antibody [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Carbohydrate Sulfotransferase 4/CHST4 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Carbohydrate Sulfotransferase 4/CHST4
  Arg30-Gly388
• Specificity: Detects mouse Carbohydrate Sulfotransferase 4/CHST4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human (rh) CHST4 is observed and less than 5% cross‑reactiv
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 4/CHST4 Antibody [Alexa Fluor® 350]

• carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
• Carbohydrate Sulfotransferase 4
• CHST4
• EC 2.8.2
• EC 2.8.2.-
• EC 2.8.2.17
• EC 2.8.2.21
• galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3
• Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3
• GlcNAc6ST2
• GlcNAc6ST-2
• gn6st-2
• GST3
• GST-3
• HECGLCNAC6ST
• HEC-GlcNAc6ST
• HEC-GLCNAC-6-ST
• High endothelial cells N-acetylglucosamine 6-O-sulfotransferase
• L-selectin ligand sulfotransferase
• LSST
• LSSTcarbohydrate sulfotransferase 4
• N-acetylglucosamine 6-O-sulfotransferase 2

Background

The mouse CHST family is comprised of 13 enzymes. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of the recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). Human CHST4, also known as high endothelial cell N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L‑selectin ligand sulfotransferase (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L‑selectin ligands (4). It has a catalytic preference for core 2-branched mucin-type O‑glycans, but also has activity toward core 3 type of O‑glycans (5). Mouse CHST4 shares 72% amino acid sequence identity with the human ortholog.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.