carabin Antibody

Western Blot: carabin Antibody [NBP2-76349] - Staining of Human Spleen (A) and Mouse Thymus (B) lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.

Flow Cytometry: carabin Antibody [NBP2-76349] - Paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: carabin Antibody [NBP2-76349] - Staining of Jurkat with antibody at 0.5 ug/mL (A), PBM with antibody at 0.3 ug/mL (B) and negative control A431 (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

carabin Antibody Summary

• Description: Novus Biologicals Goat carabin Antibody (NBP2-76349) is a polyclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PRRKPQTRGKTFH corresponding to internal region according to NP_940919.1.
• Predicted Species: Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TBC1D10C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Peptide ELISA Detection limit 1:28000
  • Western Blot
• Application Notes: WB: Preliminary experiments in human tonsil, thymus and peripheral mononucleocytes lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for carabin Antibody

• carabin
• FLJ00332
• MGC46488
• TBC1 domain family member 10C
• TBC1 domain family, member 10C

Background

Carabin is an endogenous inhibitor of calcineurin (see MIM 114105) that also inhibits the Ras (see MIM 190020) signaling pathway through its intrinsic Ras GTPase-activating protein activity (Pan et al., 2007 [PubMed 17230191]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBC1D10C