CaM Kinase II Antibody (990714) [Unconjugated]

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CaM Kinase II was detected in immersion fixed PC‑3 human prostate cancer cell line using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 8 µg/mL for 3 hours at ...read more
CaM Kinase II was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 0.5 µg/mL for 1 ...read more
CaM Kinase II was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 1.7 µg/mL for 1 ...read more
CaM Kinase II was detected in perfusion fixed frozen sections of mouse brain (brainstem) using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 1.7 µg/mL for 1 ...read more
Western blot shows lysates of human brain (hippocampus) tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal ...read more
Western blot shows lysates of recombinant human CaM Kinase II alpha , recombinant human CaM Kinase II beta , recombinant human CaM Kinase II gamma , and recombinant human CaM Kinase II δ. PVDF membrane ...read more
CaM Kinase II was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 25 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
990714
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

CaM Kinase II Antibody (990714) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CaM Kinase II gamma
Ala448-Gln558
Accession # Q13555
Specificity
Detects human CaM Kinase II gamma in direct ELISAs. Detects human CaM Kinase II alpha , beta , gamma , and δ in Western blots. Detects human, mouse, and rat CaM Kinase II in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 0.5-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB7280 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CaM Kinase II Antibody (990714) [Unconjugated]

  • CaM Kinase II
  • CAMK2
  • CAMK2B

Background

Calcium/calmodulin-dependent protein kinase type II gamma (CaMKII gamma ) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha , beta , gamma , and δ, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. The widely expressed CaMKII gamma from human, mouse, and rat share 100% aa sequence identity within aa 448-558 of isoform 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CaM Kinase II Antibody (MAB7280)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Western Blot.


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