CaM Kinase II Antibody (990714) [Unconjugated]

CaM Kinase II was detected in immersion fixed PC‑3 human prostate cancer cell line using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CaM Kinase II was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 0.5 µg/mL for 1 hour at room temperature. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CaM Kinase II was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 1.7 µg/mL for 1 hour at room temperature. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in intestinal gland cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CaM Kinase II was detected in perfusion fixed frozen sections of mouse brain (brainstem) using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 1.7 µg/mL for 1 hour at room temperature. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western blot shows lysates of human brain (hippocampus) tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CaM Kinase II at approximately 50 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of recombinant human CaM Kinase II alpha, recombinant human CaM Kinase II beta, recombinant human CaM Kinase II gamma, and recombinant human CaM Kinase II δ. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CaM Kinase II was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse/Rat CaM Kinase II Pan Specific Monoclonal Antibody (Catalog # MAB7280) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, ICC/IF
• Clone: 990714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CaM Kinase II Antibody (990714) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CaM Kinase II gamma
  Ala448-Gln558
  Accession # Q13555
• Specificity: Detects human CaM Kinase II gamma in direct ELISAs. Detects human CaM Kinase II alpha, beta, gamma, and δ in Western blots. Detects human, mouse, and rat CaM Kinase II in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 0.5-25 ug/mL
  • Immunocytochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CaM Kinase II Antibody (990714) [Unconjugated]

• CaM Kinase II
• CAMK2
• CAMK2B

Background

Calcium/calmodulin-dependent protein kinase type II gamma (CaMKII gamma ) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. The widely expressed CaMKII gamma from human, mouse, and rat share 100% aa sequence identity within aa 448-558 of isoform 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q13555(Human)