Calsenilin Overexpression Lysate

Images

 
Western Blot: CSEN Overexpression Lysate (Adult Normal) [NBL1-12160] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CSEN.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Calsenilin Overexpression Lysate Summary

Description
Transient overexpression lysate of Kv channel interacting protein 3, calsenilin (KCNIP3), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC203957 and based on accession number NM_013434. The protein contains a C-MYC/DDK Tag.
Gene
KCNIP3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
29.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Calsenilin Overexpression Lysate

  • calsenilin
  • calsenilin, presenilin-binding protein, EF hand transcription factor
  • CSEN MGC18289
  • DREAM
  • DREAMA-type potassium channel modulatory protein 3
  • KCHIP3 DRE-antagonist modulator
  • KChIP3
  • Kv channel interacting protein 3, calsenilin
  • Kv channel-interacting protein 3
  • potassium channel interacting protein 3

Background

This gene encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins, which belong to the recoverin branch of the EF-hand superfamily. Members of this family are small calcium binding proteins containing EF-hand-like domains. They are integral subunit components of native Kv4 channel complexes that may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. The encoded protein also functions as a calcium-regulated transcriptional repressor, and interacts with presenilins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Calsenilin Lysate (NBL1-12160) (0)

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Other Available Formats

NBL1-12160

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Bioinformatics

Gene Symbol KCNIP3