Calpastatin Antibody (CAST/1550) - Azide and BSA Free

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510] - Formalin-fixed, paraffin-embedded human Tonsil stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: CAST/1550
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Calpastatin Antibody (CAST/1550) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07397).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human Calpastatin protein (Uniprot: P20810)
• Localization: Cytoplasm and Cell Surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAST
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2-4 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA
• Theoretical MW: 126 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Calpastatin Antibody (CAST/1550) - Azide and BSA Free

• BS-17
• Calpain inhibitor
• calpastatin
• MGC9402
• Sperm BS-17 component

Background

The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When Calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAST