Recombinant Human Calpain 3 Protein

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SDS-Page: Recombinant Human Calpain 3 Protein [H00000825-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PA, AP

Order Details

Recombinant Human Calpain 3 Protein Summary

Description
A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 210-309 of Human Calpain 3

Source: Wheat Germ

Amino Acid Sequence: NKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Details of Functionality
This protein is not active and should not be used for experiments requiring activity.
Source
Wheat germ
Protein/Peptide Type
Partial Recombinant Protein
Gene
CAPN3
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Application Notes
This protein has not been tested for any functionality. Product may contain endotoxins and is not suitable for use with live cells.
Theoretical MW
36.74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Calpain 3 Protein

  • calpain 3, (p94)
  • Calpain L3
  • Calpain p94
  • calpain, large polypeptide L3
  • calpain-3
  • CANP 3
  • CANP3MGC4403
  • CANPL3
  • EC 3.4.22
  • EC 3.4.22.54
  • LGMD2
  • LGMD2A
  • MGC10767
  • MGC11121
  • MGC14344
  • muscle-specific calcium-activated neutral protease 3 large subunit
  • Muscle-specific calcium-activated neutral protease 3
  • NCL1
  • nCL-1large [catalytic] subunit
  • New calpain 1

Background

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CAPN3