Cadherin-13 Overexpression Lysate

Western Blot: H Cadherin Overexpression Lysate (Adult Normal) [NBL1-09026] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H Cadherin.

• Reactivity: Hu
• Applications: WB

Cadherin-13 Overexpression Lysate Summary

• Description: Transient overexpression lysate of cadherin 13, H-cadherin (heart) (CDH13) The lysate was created in HEK293T cells, using Plasmid ID RC206068 and based on accession number NM_001257. The protein contains a C-MYC/DDK Tag.
• Gene: CDH13

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 75.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cadherin-13 Overexpression Lysate

• cadherin 13, H-cadherin (heart)
• Cadherin13
• Cadherin-13
• CDH13
• CDHHT-cadherin
• H-Cadherin
• Heart cadherin
• heart-cadherin
• P105
• T-cad
• T-Cadherin
• Truncated cadherin
• truncated-cadherin

Background

This gene is a member of the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth. The gene locus is hypermethylated or deleted in breast, ovarian and lung cancers. Two major mRNA transcripts encoding identical proteins are found, products of alternative polyadenylation sites. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CDH13