CACYBP Overexpression Lysate (Native)

Images

 
Western Blot: CACYBP Overexpression Lysate (Adult Normal) [NBL1-08636] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CACYBP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CACYBP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203302 and based on accession number NM_014412. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens calcyclin binding protein (CACYBP), transcript variant 1, mRNA.
Gene
CACYBP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CACYBP

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CACYBP Overexpression Lysate (Native)

  • CacyBP
  • calcyclin binding protein
  • GIG5
  • hCacyBP
  • MGC87971
  • RP1-102G20.6
  • S100A6-binding protein
  • S100A6BPgrowth-inhibiting gene 5 protein
  • Siah-interacting protein (SIP)
  • Siah-interacting protein
  • SIPcalcyclin-binding protein

Background

The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89388
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NBP2-45224
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-37539
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-29403
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-91258
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB300-872
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA
MAB4605
Species: Hu
Applications: WB, Simple Western, ICC, ICFlow
NBP1-82991
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-24915
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P
H00008720-M07
Species: Hu
Applications: ELISA, ICC/IF
AF1436
Species: Mu
Applications: WB
NBP2-38518
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-541
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
NB100-56605
Species: Av, Bv, Sh
Applications: WB

Publications for CACYBP Lysate (NBL1-08636) (0)

There are no publications for CACYBP Lysate (NBL1-08636).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CACYBP Lysate (NBL1-08636) (0)

There are no reviews for CACYBP Lysate (NBL1-08636). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CACYBP Lysate (NBL1-08636) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CACYBP Products

Bioinformatics Tool for CACYBP Lysate (NBL1-08636)

Discover related pathways, diseases and genes to CACYBP Lysate (NBL1-08636). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CACYBP Lysate (NBL1-08636)

Discover more about diseases related to CACYBP Lysate (NBL1-08636).
 

Pathways for CACYBP Lysate (NBL1-08636)

View related products by pathway.

PTMs for CACYBP Lysate (NBL1-08636)

Learn more about PTMs related to CACYBP Lysate (NBL1-08636).
 

Research Areas for CACYBP Lysate (NBL1-08636)

Find related products by research area.

Blogs on CACYBP

There are no specific blogs for CACYBP, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CACYBP Antibody
NBP1-87104

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CACYBP Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CACYBP