CACNG3 Antibody

Western Blot: CACNG3 Antibody [NBP2-76918] - Western blot analysis of CACNG3 on SH-SY-5Y cell lysates using anti-CACNG3 antibody.Lane 1: Anti-CACNG3 Antibody (1:500). Lane 2: Anti-CACNG3 Antibody, pre-incubated with the immunizaiton peptide.

Immunocytochemistry/Immunofluorescence: CACNG3 Antibody [NBP2-76918] - ICC staining CACNG3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: CACNG3 Antibody [NBP2-76918] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNG3 antibody. Counter stained with hematoxylin.

Flow Cytometry: CACNG3 Antibody [NBP2-76918] - Flow cytometric analysis of SH-SY-5Y cells with CACNG3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Discontinued

CACNG3 Antibody Summary

• Immunogen: Synthetic peptide within rat CACNG3 aa 192-241 / 315. (SwissProt: O60359 Human; SwissProt: Q8VHX0 Rat)
• Localization: Membrane. Displays a somatodendritic localization and is excluded from axons in neurons.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CACNG3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CACNG3 Antibody

• calcium channel, voltage-dependent, gamma subunit 3
• Neuronal voltage-gated calcium channel gamma-3 subunit
• voltage-dependent calcium channel gamma-3 subunit
• voltage-gated calcium channel gamma subunit

Background

L-type calcium channels are composed of five subunits. The protein encoded by this gene represents one of these subunits, gamma, and is one of several gamma subunit proteins. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This protein is similar to the mouse stargazin protein, mutations in which have been associated with absence seizures, also known as petit-mal or spike-wave seizures. This gene is a member of the neuronal calcium channel gamma subunit gene subfamily of the PMP-22/EMP/MP20 family. This gene is a candidate gene for a familial infantile convulsive disorder with paroxysomal choreoathetosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACNG3