CACNA2D2 Antibody (N492/39) [FL490] - Chimeric

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clone: N492/39
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: FL490
• Concentration: 0.5 mg/ml

CACNA2D2 Antibody (N492/39) [FL490] - Chimeric Summary

• Description: FL490 Excitation: 491 nm, Emission: 515nm.
  Each order is a unique lot. For specialized requirements, please inquire for available options.
  
  This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N492/39 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N492/39 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein amino acids 61-197 (extracellular domain) of mouse CACNA2D2 (Uniprot#: Q6PHS9) produced recombinantly in E.Coli.
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: CACNA2D2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 1X PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for CACNA2D2 Antibody (N492/39) [FL490] - Chimeric

• alpha 2 delta calcium channel subunit
• CACNA2D
• CACNA2D2
• calcium channel, voltage-dependent, alpha 2/delta subunit 2
• gene 26
• KIAA0558
• KIAA0558Voltage-gated calcium channel subunit alpha-2/delta-2
• LUAC11.1
• voltage-dependent calcium channel subunit alpha-2/delta-2

Background

The CACNA2D2 gene encodes a voltage-dependent calcium channel subunit alpha-2/delta-2 protein that exists in 5 isoforms: isoform 1: 1,150 amino acids long, 129 kDA; isoform 2: 1,143 amino acids long, 129 kDA; isoform 3: 1,145 amino acids long, 129 kDA; isoform 4: 1,076 amino acids long, 122 kDA; and isoform 5: 1,152 amino acids long, 130 kDA. These proteins are critical in calcium current density and in activation and inactivation kinetics of the calcium channel. Additionally, these proteins function as regulatory subunits for P/Q-type calcium channel, N-type, and L-type, as well as T-type. Overexpression of this gene triggers apoptosis. The CACNA2D2 gene participates in transcription of the CREB pathway, the caspase cascade, Fc-GammaR pathway, BMP and PDGF pathways, regulation of insulin secretion, and transmission across chemical synapses. It is known to interact with genes DLG4, CACNA1C, CACNA1D, CACNA1F, and YWHAB. The CACNA2D2 gene is associated with ataxia, seizures, lung cancer, and neuronitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACNA2D2