CABP1 Antibody (JE56-72)

Western Blot: CABP1 Antibody (JE56-72) [NBP3-32099] - Western blot analysis of CABP1 on different lysates with Rabbit anti-CABP1 antibody (NBP3-32099) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 ug/Lane) Lane 2: Human brain tissue lysate (40 ug/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32099) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: CABP1 Antibody (JE56-72) [NBP3-32099] - Flow cytometric analysis of U-87 MG cells labeling CABP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32099, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu
• Applications: WB, Flow
• Clone: JE56-72
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CABP1 Antibody (JE56-72) Summary

• Description: Novus Biologicals Rabbit CABP1 Antibody (JE56-72) (NBP3-32099) is a recombinant monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human CABP1 aa 150-250. (Uniprot: Q9NZU7)
• Localization: Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Synapse.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CABP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Western Blot 1:1000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CABP1 Antibody (JE56-72)

• CaBP1
• CALBRAIN
• calcium binding protein 1
• calcium binding protein 5
• calcium-binding protein 1
• caldendrin
• HCALB_BR

Background

CABP1 belongs to a subfamily of calcium binding proteins, which share similarity to calmodulin. Calcium binding proteins are an important component of calcium mediated cellular signal transduction. This protein is only detected in retina and brain. Study of the mouse homolog demonstrated that groups of cells expressing this protein are located in the center or inner border of the inner unclear layer of retina. Two alternatively spliced variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CABP1