BTNL2/Butyrophilin-like 2 Antibody [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

BTNL2/Butyrophilin-like 2 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse BTNL2/Butyrophilin-like 2
  aa 27-452
  Accession # O70355
• Specificity: Detects mouse BTNL2/Butyrophilin-like 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) BTN, rmBTN2, and recombinant human BTN is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTNL2/Butyrophilin-like 2 Antibody [Alexa Fluor® 350]

• BTL-II
• BTNL2
• Butyrophilin like 2
• butyrophilin-like 2 (MHC class II associated)
• Butyrophilin-like 2

Background

Butyrophilin-like 2 (BTNL2; also BTL-II) is a predicted 60‑65 kDa member of the BTN/MOG family, Ig-superfamily of proteins. In mouse, it is expressed in intestinal villi columnar epithelial cells and colonic dendritic cells. BTNL2 apparently blocks CD4+ T cell activation. Mouse BTNL2 is a 454 amino acid (aa) type II transmembrane glycoprotein. It contains a 431 aa extracellular region (aa 24‑454) with four V-type Ig-like domains. There are three splice variants. One shows a deletion of the two C-terminal amino acids, a second shows a deletion of aa 237‑243, while a third shows a C-terminal 62 substitution for Gly453Trp454. Over aa 27‑452, mouse BTNL2 shares 88% and 64% aa identity with rat and human BTNL2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.