BTG3 Overexpression Lysate (Native)

Images

 
Western Blot: BTG3 Overexpression Lysate (Adult Normal) [NBL1-08049] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BTG3.

Product Details

Summary
Applications WB

Order Details

BTG3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221056 and based on accession number NM_006806. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens BTG family, member 3 (BTG3), transcript variant 2, mRNA.
Gene
BTG3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for BTG3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BTG3 Overexpression Lysate (Native)

  • Abundant in neuroepithelium area protein
  • ANATOB55
  • B-cell translocation gene 3
  • B-cell translocation gene10
  • BTG family member 3
  • BTG family, member 3
  • MGC8928
  • protein BTG3
  • Protein Tob5
  • tob55
  • TOB5TOFA

Background

The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein might play a role in neurogenesis in the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for BTG3 Lysate (NBL1-08049) (0)

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Bioinformatics

Gene Symbol BTG3