BST2 Antibody (2E6)

Western Blot: BST2 Antibody (2E6) [H00000684-M15] - Analysis of BST2 expression in human placenta.

Immunocytochemistry/Immunofluorescence: BST2 Antibody (2E6) [H00000684-M15] - Analysis of monoclonal antibody to BST2 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: BST2 Antibody (2E6) [H00000684-M15] - Analysis of BST2 expression in HeLa.

Immunoprecipitation: BST2 Antibody (2E6) [H00000684-M15] - Analysis of BST2 transfected lysate using anti-BST2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BST2 rabbit polyclonal antibody.

Sandwich ELISA: BST2 Antibody (2E6) [H00000684-M15] - Detection limit for recombinant GST tagged BST2 is 0.1 ng/ml as a capture antibody.

Product Discontinued

BST2 Antibody (2E6) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: BST2 (AAH33873.1, 1 a.a. ~ 180 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASTSYDYCRVPMEDGDKRCKLLLGIGILVLLIIVILGVPLIIFTIKANSEACRDGLRAVMECRNVTHLLQQELTEAQKGFQDVEAQAATCNHTVMALMASLDAEKAQGQKKVEELEGEITTLNHKLQDASAEVERLRRENQVLSVRIADKKYYPSSQDSSSAAAPQLLIVLLGLSALLQ
• Specificity: BST2 - bone marrow stromal cell antigen 2 (2E6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BST2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BST2 Antibody (2E6)

• bone marrow stromal antigen 2
• bone marrow stromal cell antigen 2
• BST2
• BST-2
• CD317 antigen
• CD317
• HM1.24 Antigen
• NPC-A-7
• PDCA-1
• Tetherin

Background

Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BST2 Antibody (H00000684-M15)(4)

Publications using H00000684-M15

• Liu Y, Li M, Zhang D et al. HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway. Virol J 2016-09-15 [PMID: 27630089] (WB)
• Lv M, Wang J, Zhang J et al. Epitope Tags beside the N-Terminal Cytoplasmic Tail of Human BST-2 Alter Its Intracellular Trafficking and HIV-1 Restriction. PLoS One. 2014-10-27 [PMID: 25347789]
• Lv M, Zhu Y, Wang J et al. Purification of eukaryotic tetherin/Vpu proteins and detection of their interaction by ELISA. Protein Expr Purif. 2013-08-03 [PMID: 23916489]
• Lv M, Wang J, Wang X et al. Polarity Changes in the Transmembrane Domain Core of HIV-1 Vpu Inhibits Its Anti-Tetherin Activity. PLoS One. 2011-06-02 [PMID: 21674066]

Bioinformatics

• Gene Symbol: BST2
• Entrez:
  • 684(Human)
• Uniprot:
  • AAH33873(Human)