BNIP2 Overexpression Lysate

Western Blot: BNIP2 Overexpression Lysate (Adult Normal) [NBL1-08013] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BNIP2.

Western Blot: BNIP2 Overexpression Lysate (Adult Normal) [NBL1-08013] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for BNIP2

• Reactivity: Hu
• Applications: WB

BNIP2 Overexpression Lysate Summary

• Description:

  BNIP2 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC200377
  
  Accession#: NM_004330
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: BNIP2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 48.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BNIP2 Overexpression Lysate

• BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
• BCL2/adenovirus E1B 19kDa interacting protein 2
• BCL2/adenovirus E1B 19kD-interacting protein 2
• BNIP-2
• NIP2

Background

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. Though the specific function is unknown, it interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. [provided by RefSeq]. Sequence Note: The translation start codon selected for this RefSeq is conserved in human and Rhesus macaque. A possible downstream start codon, which shows better cross-species conservation, would shorten the protein by 121 aa. The shorter protein is described in the literature, including PMID:7954800. No experimental evidence exists to show which start codon is used in vivo. CCDS Note: The coding region has been updated to extend the N-terminus based on the presence of an upstream translation start codon, which is conserved in human and Rhesus macaque. A possible downstream start codon, which shows better cross-species conservation, would shorten the protein by 121 aa. The shorter protein is described in the literature, including PMID:7954800. No experimental evidence exists to show which start codon is used in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: BNIP2