BNIP2 Overexpression Lysate (Native)

Images

 
Western Blot: BNIP2 Overexpression Lysate (Adult Normal) [NBL1-08013] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BNIP2.
Western Blot: BNIP2 Overexpression Lysate (Adult Normal) [NBL1-08013] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for BNIP2

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

BNIP2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200377 and based on accession number NM_004330. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens BCL2/adenovirus E1B 19kDa interacting protein 2 (BNIP2), mRNA.
Gene
BNIP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
48.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for BNIP2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BNIP2 Overexpression Lysate (Native)

  • BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
  • BCL2/adenovirus E1B 19kDa interacting protein 2
  • BCL2/adenovirus E1B 19kD-interacting protein 2
  • BNIP-2
  • NIP2

Background

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. Though the specific function is unknown, it interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. [provided by RefSeq]. Sequence Note: The translation start codon selected for this RefSeq is conserved in human and Rhesus macaque. A possible downstream start codon, which shows better cross-species conservation, would shorten the protein by 121 aa. The shorter protein is described in the literature, including PMID:7954800. No experimental evidence exists to show which start codon is used in vivo. CCDS Note: The coding region has been updated to extend the N-terminus based on the presence of an upstream translation start codon, which is conserved in human and Rhesus macaque. A possible downstream start codon, which shows better cross-species conservation, would shorten the protein by 121 aa. The shorter protein is described in the literature, including PMID:7954800. No experimental evidence exists to show which start codon is used in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol BNIP2