Recombinant Human Biliverdin Reductase A/BLVRA Protein

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SDS-Page: Biliverdin Reductase A Protein [NBP1-30216] - Biliverdin Reductase A Protein Biliverdin reductase A, 33.3 kDa (295aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Biliverdin Reductase A/BLVRA Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 3-296 of Human Biliverdin Reductase A/BLVRA

Source: E.coli

Amino Acid Sequence: MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK

Details of Functionality
Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
BLVRA
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
33.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Biliverdin Reductase A/BLVRA Protein

  • Biliverdin Reductase A
  • Biliverdin-IX alpha-reductase
  • BLVR
  • BLVRA
  • BVR A
  • BVR
  • BVRA
  • EC 1.3.1.24

Background

BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond.( Bilirubin + NAD(P)+ = biliverdin + NAD(P)H ) Recombinant BLVRA protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol BLVRA
Entrez