Recombinant Human Biliverdin Reductase A/BLVRA Protein

Product Discontinued

Recombinant Human Biliverdin Reductase A/BLVRA Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 3-296 of Human Biliverdin Reductase A/BLVRA
  
  

  Source: E.coli

  Amino Acid Sequence: MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK

• Details of Functionality: Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BLVRA
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 33.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Biliverdin Reductase A/BLVRA Protein

• Biliverdin Reductase A
• Biliverdin-IX alpha-reductase
• BLVR
• BLVRA
• BVR A
• BVR
• BVRA
• EC 1.3.1.24

Background

BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond.( Bilirubin + NAD(P)+ = biliverdin + NAD(P)H ) Recombinant BLVRA protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BLVRA