BHMT2 Antibody

Western Blot: BHMT2 Antibody [NBP2-15585] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: BHMT2 Antibody [NBP2-15585] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Western Blot: BHMT2 Antibody [NBP2-15585] - Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE gel, diluted at 1:20000.

Product Discontinued

BHMT2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human BHMT2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BHMT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:1000-1:20000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (89%), Rat (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for BHMT2 Antibody

• betaine-homocysteine methyltransferase 2
• betaine--homocysteine S-methyltransferase 2
• EC 2.1.1.5
• FLJ20001

Background

Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for BHMT2 Antibody (NBP2-15585). (Showing 1 - 1 of 1 FAQ).

1. I was looking at the BHMT2 antibody IHC image in brain tissue and I want to know where is this protein expressed? Cytosolic or nuclear ? the image you are showing does not have details.
   • BHMT2 protein is not a very well published target and I do not see any research paper with its immuno-staining data. However, our antibodies NBP1-85826 and NBP2-15585 depicted a strong cytoplasmic staining with mild nuclear positivity for this target in IHC-P and ICC-IF assays. Human Protein Atlas also shows mainly cytoplasmic staining in hepatocytes, renal tubules, thyroid gland and a subset of cells in exocrine pancreas. Chadwick et al. (Genomics. 2000 Nov 15;70(1):66-73) reported that BHMT2 is expressed in liver and kidney, and at reduced levels in the brain, heart, and skeletal muscle.

Bioinformatics

• Gene Symbol: BHMT2
• Uniprot:
  • Q9H2M3(Human)