Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody

Western blot shows lysates of human brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2
  Lys29-Cys397
  Accession # Q9NY97
• Specificity: Detects human and rat beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: B3GNT2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody

• B3Galt6
• B3GALT7
• b3Gal-T7
• B3GNT
• B3GNT1
• B3GN-T1
• B3GNT2
• B3GN-T2
• B3GNT-2
• Beta-1,3-galactosyltransferase 7
• Beta-1,3-GalTase 7
• Beta-1,3-Gn-T1
• Beta-1,3-Gn-T2
• Beta-1,3-N-acetylglucosaminyltransferase 1
• Beta-1,3-N-acetylglucosaminyltransferase 2
• beta-1,3-N-acetylglucosaminyltransferase bGnT-1
• beta-1,3-N-acetylglucosaminyltransferase bGnT-2
• Beta3GalT7
• Beta3Gal-T7
• BETA3GNT
• Beta3gnt1
• Beta3Gn-T1
• Beta3Gn-T2
• Beta-3-Gx-T7
• BGnT-1
• BGNT2
• BGnT-2
• EC 2.4.1.-
• UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7
• UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2

Background

B3-GNT (also B3GNT-2) is an 80 kDa (45 kDa without glycosylation) type II transmembrane  Golgi enzyme that belongs to the glycosyltransferase 31 family of enzymes. It catalyzes the transfer of N-acetyl glucosamine from UDP-GlcNAc onto Gal beta 3(GlcNAc beta 6)GalNAc-mucin. It functions in the elongation and branching of O-linked oligosaccharide chains of mucin glycoproteins. Over aa 29-397, human B3GNT2 shares 88% aa sequence identity with rat B3GNT2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B3GNT2
• Uniprot:
  • Q9NY97()