Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (485327)

• Reactivity: Hu
• Applications: WB
• Clone: 485327
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (485327) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2
  Lys29-Cys397
  Accession # Q9NY97
• Specificity: Detects human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human B4GALT1 is observed
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: B3GNT2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (485327)

• B3Galt6
• B3GALT7
• b3Gal-T7
• B3GNT
• B3GNT1
• B3GN-T1
• B3GNT2
• B3GN-T2
• B3GNT-2
• Beta-1,3-galactosyltransferase 7
• Beta-1,3-GalTase 7
• beta-1,3-Gn-T1
• beta-1,3-Gn-T2
• Beta-1,3-N-acetylglucosaminyltransferase 1
• Beta-1,3-N-acetylglucosaminyltransferase 2
• beta-1,3-N-acetylglucosaminyltransferase bGnT-1
• beta-1,3-N-acetylglucosaminyltransferase bGnT-2
• Beta3GalT7
• Beta3Gal-T7
• BETA3GNT
• Beta3gnt1
• Beta3Gn-T1
• Beta3Gn-T2
• Beta-3-Gx-T7
• BGnT-1
• BGNT2
• BGnT-2
• EC 2.4.1.-
• UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7
• UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2

Background

beta 1,3-Linked GlcNAc residues are present in the backbone of various biologically important glycans which are involved in many essential biological functions such as keratan sulfate synthesis in corneal tissue (1). The addition of such residues are catalyzed by a family of beta 1,3‑N‑acetylglucosaminyltransferases, that includes at least eight members (1‑5).  All of them are type II Golgi resident transmembrane proteins and have high homology to the beta 1,3-galactosyltransferase family. beta 1,3‑N‑acetylglucosaminyltransferases 2 or beta 3GNT2 prefers the substrate lacto-N-neotetraose and is involved in the biosynthesis of poly-N-acetyllactosamine chains (6).

1. Seko, A. and Yamashita, K. (2005) Glycobiology 15:943.
2. Kataoka, K. and Huh, N.-H. (2002) Biochem. Biophys. Res. Commun. 294:843.
3. Iwai,T. et al. (2002) J. Biol. Chem. 277:12802.
4. Togayachi, A. et al. (2001) J. Biol. Chem. 276:22032.
5. Sasaki, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14294.
6. Shiraishi, N. et al. (2001) J. Biol. Chem. 276:3498.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B3GNT2
• Uniprot:
  • Q9NY97()