Bestrophin 3 Antibody (OTI2H3)

Western Blot: Bestrophin 3 Antibody (2H3) [NBP2-03253] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Bestrophin 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Bestrophin 3.

Immunocytochemistry/Immunofluorescence: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Bestrophin 3.

Flow Cytometry: Bestrophin 3 Antibody (2H3) [NBP2-03253] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Bestrophin 3 antibody, and then analyzed by flow cytometry.

Flow Cytometry: Bestrophin 3 Antibody (2H3) [NBP2-03253] - Analysis of Hela cells, using anti-Bestrophin 3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI2H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.98 mg/ml

Bestrophin 3 Antibody (OTI2H3) Summary

• Description: Novus Biologicals Mouse Bestrophin 3 Antibody (OTI2H3) (NBP2-03253) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human BEST3 (NP_689652) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BEST3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 50.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.98 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Bestrophin 3 Antibody (OTI2H3)

• bestrophin 3
• bestrophin-3
• MGC13168
• MGC40411
• vitelliform macular dystrophy 2-like 3
• VMD2L3Vitelliform macular dystrophy 2-like protein 3

Background

BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BEST3
• Entrez:
  • 144453(Human)