Recombinant Human BCAT2 Protein Summary
| Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-392 of Human BCAT2 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASSSF KAADLQLEMT QKPHKKPGPG EPLVFGKTFT DHMLMVEWND KGWGQPRIQP FQNLTLHPAS SSLHYSLQLF EGMKAFKGKD QQVRLFRPWL NMDRMLRSAM RLCLPSFDKL ELLECIRRLI EVDKDWVPDA AGTSLYVRPV LIGNEPSLGV SQPTRALLFV ILCPVGAYFP GGSVTPVSLL ADPAFIRAWV GGVGNYKLGG NYGPTVLVQQ EALKRGCEQV LWLYGPDHQL TEVGTMNIFV YWTHEDGVLE LVTPPLNGVI LPGVVRQSLL DMAQTWGEFR VVERTITMKQ LLRALEEGRV REVFGSGTAC QVCPVHRILY KDRNLHIPTM ENGPELILRF QKELKEIQYG IRAHEWMFPV |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
BCAT2 |
| Purity |
>90%, by SDS-PAGE |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
43.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20 mM Tris-HCl buffer (pH 7.5), 10% glycerol, 0.2 M NaCl, 1 mM DTT |
| Preservative |
No Preservative |
| Concentration |
0.5 mg/ml |
| Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant Human BCAT2 Protein
Background
BCAT2 belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. This protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. It may also function as a transporter of branched chain alpha-keto acids. Recombinant human BCAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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