Recombinant Human BCAS2 His Protein

SDS-Page: Recombinant Human BCAS2 Protein [NBP1-50992] - 15 % SDS-PAGE (3ug)

Product Discontinued

Recombinant Human BCAS2 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-225 of Human BCAS2
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAGT GLVAGEVVVD ALPYFDQGYE APGVREAAAA LVEEETRRYR PTKNYLSYLT APDYSAFETD IMRNEFERLA ARQPIELLSM KRYELPAPSS GQKNDITAWQ ECVNNSMAQL EHQAVRIENL ELMSQHGCNA WKVYNENLVH MIEHAQKELQ KLRKHIQDLN WQRKNMQLTA GSKLREMESN WVSLVSKNYE IERTIVQLEN EIYQIKQQHG EANKENIRQD F

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BCAS2
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 30.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT, 0.2 M NaCl
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human BCAS2 His Protein

• breast carcinoma amplified sequence 2
• Breast carcinoma-amplified sequence 2
• DAM1DNA amplified in mammary carcinoma 1 protein
• pre-mRNA-splicing factor SPF27
• Snt309
• SPF27
• spliceosome associated protein, amplified in breast cancer
• Spliceosome-associated protein SPF 27

Background

BCAS2, also known as pre-mRNA-splicing factor SPF27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. It is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TRbeta (thyroid hormone receptor beta), ERalpha (estrogen receptor alpha), ERbeta, PR (progesterone receptor) and PPARgamma (peroxisome proliferator-activated receptor gamma). This protein functions as an ER co-activator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer. Recombinant human BCAS2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BCAS2