BCAM/CD239 Antibody (JE51-07)

Western Blot: BCAM/CD239 Antibody (JE51-07) [NBP2-80450] - Analysis of BCAM/CD239 on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80450, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: BCAM/CD239 Antibody (JE51-07) [NBP2-80450] - Analysis in human placenta tissue using BCAM/BCAM/CD239 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, w

Flow Cytometry: BCAM/CD239 Antibody (JE51-07) [NBP2-80450] - Analysis of CD239 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: BCAM/CD239 Antibody (JE51-07) [NBP2-80450] - Analysis in human kidney tissue using BCAM/BCAM/CD239 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, was

Product Discontinued

BCAM/CD239 Antibody (JE51-07) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human BCAM/CD239 aa 579-628 / 628. (SwissProt: P50895 Human)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BCAM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:2000
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BCAM/CD239 Antibody (JE51-07)

• antigen identified by monoclonal F8
• Auberger B antigen
• basal cell adhesion molecule (Lu and Au blood groups)
• basal cell adhesion molecule (Lutheran blood group)
• basal cell adhesion molecule
• B-CAM cell surface glycoprotein
• BCAM
• B-cell adhesion molecule
• CD239 antigen
• CD239
• F8/G253 antigen
• glycoprotein 95kDa
• LUAU
• Lutheran antigen
• Lutheran blood group (Auberger b antigen included)
• Lutheran blood group glycoprotein
• MSK19

Background

BCAM - Lutheran blood group (Auberger b antigen included)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCAM