BASP1 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

BASP1 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human BASP1
  Gly2-Ala45
  Accession # P80723
• Specificity: Detects human BASP1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BASP1 Antibody [Alexa Fluor® 405]

• BASP1
• brain abundant, membrane attached signal protein 1,22 kDa neuronal tissue-enriched acidic protein
• brain acid-soluble protein 1
• CAP23
• CAP-23
• CAP23brain acid soluble protein 1
• NAP22
• NAP-22
• NAP22MGC8555
• Neuronal axonal membrane protein NAP-22
• neuronal tissue-enriched acidic protein

Background

BASP1 (Brain acid soluble protein 1; also NAP-22) is a transcriptional cosuppressor that belongs to the BASP1 family of proteins. Although its predicted MW is 23 kDa, BASP1 will show multiple bands in SDS-Page. First, BASP1 forms oligomers in SDS, ranging in size from 30-150 kDa. Second, varying concentrations of SDS will change the apparent BASP1 MW from 56 kDa in 8% SDS-Page to 41 kDa in 13% SDS-Page. At least two forms of BASP1 apparently exist in human, one that is 48-52 kDa, and another that is 32-40 kDa in size. They are not functionally equivalent. BASP1 can be SUMOylated that add 20 kDa in apparent MW. There are also N-terminal fragments termed BIRPs that run from 30-50 kDa in 12% acidic SDS-Page. BASP1 is expressed in neurons, renal podocytes and spermatids. It binds to the cytoplasmic side of the plasmalemma via its myristroylate adduct, and undergoes nuclear translocation when SUMOylated. Human BASP1 is 227 amino acids (aa) in length. It contains a myristoylation site at Gly2, SUMOylation sites at Lys79 and 84, at least eight utilized phosphorylation sites, and an NLS. One splice variant shows a deletion of aa 88-141. Over aa 1-45, human BASP1 shares 96% and 91% aa identity with mouse and rat BASP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.