BACE-1 Antibody (2C1) - Azide and BSA Free

Western Blot: BACE-1 Antibody (2C1) [H00023621-M01] - BACE1 monoclonal antibody (M02), clone 2C1. Analysis of BACE1 expression in rat testis.

Immunohistochemistry-Paraffin: BACE-1 Antibody (2C1) [H00023621-M01] - Analysis of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Western Blot: BACE-1 Antibody (2C1) [H00023621-M01] - Analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate(52.9 KDa).Lane 2: Non-transfected lysate.

ELISA: BACE-1 Antibody (2C1) [H00023621-M01] - Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.

Proximity Ligation Assay: BACE-1 Antibody (2C1) [H00023621-M01] - Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Func, IHC
• Clone: 2C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BACE-1 Antibody (2C1) - Azide and BSA Free Summary

• Immunogen: BACE1 (AAH65492, 22 a.a. ~ 501 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK
• Specificity: BACE1 - beta-site APP-cleaving enzyme 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BACE1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BACE-1 Antibody (2C1) - Azide and BSA Free

• APP beta-secretase
• ASP2
• Aspartyl protease 2
• BACE1
• BACE-1
• BACEAsp 2
• beta-secretase 1 precursor variant 1
• beta-secretase 1
• beta-site amyloid beta A4 precursor protein-cleaving enzyme
• Beta-site amyloid precursor protein cleaving enzyme 1
• Beta-site APP cleaving enzyme 1
• beta-site APP-cleaving enzyme 1
• beta-site APP-cleaving enzyme
• EC 3.4.23
• EC 3.4.23.46
• FLJ90568
• HSPC104
• KIAA1149
• memapsin-2
• Membrane-associated aspartic protease 2
• transmembrane aspartic proteinase Asp2

Background

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BACE1
• Entrez:
  • 23621(Human)
• OMIM:
  • 604252(Human)
• Uniprot:
  • AAH65492(Human)