B4GALT4 Antibody (5E2) - Azide and BSA Free

Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01] - Analysis of B4GALT4 expression in transfected 293T cell line by B4GALT4 monoclonal antibody (M01), clone 5E2.Lane 1: B4GALT4 transfected lysate(40 KDa).Lane 2: Non-transfected lysate.

Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01] - Analysis of B4GALT4 over-expressed 293 cell line, cotransfected with B4GALT4 Validated Chimera RNAi ( Cat # H00008702-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B4GALT4 monoclonal antibody (M01), clone 5E2 (Cat # H00008702-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: B4GALT4 Antibody (5E2) [H00008702-M01] - Detection limit for recombinant GST tagged B4GALT4 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 5E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

B4GALT4 Antibody (5E2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: B4GALT4 (NP_003769, 35 a.a. ~ 134 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VGAIQEIPKAKEFMANFHKTLILGKGKTLTNEASTKKVELDNCPSVSPYLRGQSKLIFKPDLTLEEVQAENPKVSRGRYRPQECKALQRVAILVPHRNRE
• Specificity: B4GALT4 - UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: B4GALT4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for B4GALT4 Antibody (5E2) - Azide and BSA Free

• B4Gal-T4
• beta-1,4-galactosyltransferase 4
• Beta-1,4-GalTase 4
• beta4Gal-T4
• beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4
• EC 2.4.1.-
• UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4
• UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
• UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
• UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4

Background

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B4GALT4
• Entrez:
  • 8702(Human)
• OMIM:
  • 604015(Human)
• Uniprot:
  • NP_003769(Human)