Axotrophin Antibody

Immunohistochemistry-Paraffin: Axotrophin Antibody [NB100-93417] - (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Axotrophin Antibody Summary

• Description: Novus Biologicals Goat Axotrophin Antibody (NB100-93417) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Axotrophin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NFNQESEGRNT corresponding to internal region according to NP_073737.1.
• Epitope: C-NFNQESEGRNT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MARCH7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1ug/ml
• Application Notes: Western blot: Preliminary experiments gave an approx 125kDa band in Human Spleen and Tonsil lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.1kDa according to NP_073737.1. The 125kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Uterus.

Reactivity Notes

Expected from sequence similarity: Mouse and Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Axotrophin Antibody

• AXO
• AXOT
• Axotrophin
• E3 ubiquitin-protein ligase MARCH7
• EC 6.3.2
• EC 6.3.2.-
• MARCH-VIIDKFZp586F1122
• membrane-associated ring finger (C3HC4) 7
• Membrane-associated RING finger protein 7
• Membrane-associated RING-CH protein VII
• RING finger protein 177
• RNF177axotrophin

Background

E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligasesaccept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer theubiquitin to targeted substrates

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axotrophin Antibody (NB100-93417)(1)

Publication using NB100-93417

• Muthukumarana PA, Lyons GE, Miura Y et al. Evidence for functional inter-relationships between FOXP3, leukaemia inhibitory factor, and axotrophin/MARCH-7 in transplantation tolerance. Int Immunopharmacol 2006-12-20 [PMID: 17161353]

Bioinformatics

• Gene Symbol: MARCH7
• Entrez:
  • 64844(Human)
  • 57438(Mouse)
  • 311059(Rat)
• Uniprot:
  • NP_073737.1(Human)