Axin-1 Overexpression Lysate (Native)

Images

 
Western Blot: Axin 1 Overexpression Lysate (Adult Normal) [NBP2-04933] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Axin 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Axin-1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_851393. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Axin 1, mRNA.
Gene
AXIN1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
91.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Axin-1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Axin-1 Overexpression Lysate (Native)

  • axin 1
  • Axin1
  • Axin-1
  • AXINaxis inhibitor 1
  • Axis inhibition protein 1
  • fused, mouse, homolog of
  • hAxin
  • MGC52315

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
AF4885
Species: Mu
Applications: WB, IP
NBP1-76526
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC
NBP1-47470
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB4605
Species: Hu
Applications: WB, Simple Western, ICC, ICFlow
H00006934-M06
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
NBP1-86960
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF1620
Species: Mu
Applications: WB, IHC
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
AF1505
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP2-24648
Species: Hu, Mu, Rt, Pm
Applications: WB
NB100-1886
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for Axin-1 Lysate (NBP2-04933) (0)

There are no publications for Axin-1 Lysate (NBP2-04933).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Axin-1 Lysate (NBP2-04933) (0)

There are no reviews for Axin-1 Lysate (NBP2-04933). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Axin-1 Lysate (NBP2-04933) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Axin-1 Products

Bioinformatics Tool for Axin-1 Lysate (NBP2-04933)

Discover related pathways, diseases and genes to Axin-1 Lysate (NBP2-04933). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Axin-1 Lysate (NBP2-04933)

Discover more about diseases related to Axin-1 Lysate (NBP2-04933).
 

Pathways for Axin-1 Lysate (NBP2-04933)

View related products by pathway.

PTMs for Axin-1 Lysate (NBP2-04933)

Learn more about PTMs related to Axin-1 Lysate (NBP2-04933).
 

Research Areas for Axin-1 Lysate (NBP2-04933)

Find related products by research area.

Blogs on Axin-1

There are no specific blogs for Axin-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

c-Myc Antibody
NB600-335

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Axin-1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol AXIN1
Entrez