Axin-1 Antibody

Immunohistochemistry: Axin-1 Antibody [NB100-1011] - (3.8ug/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Axin-1 Antibody Summary

• Immunogen: Peptide with sequence C-FEEKIIGKVEKVD corresponding to C-Terminus according to NP_851393.1.
• Specificity: This antibody is expected to recognise both reported isoforms.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AXIN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments gave bands at approx. 150 kDa band and 22-24 kDa band in human brain (hippocampus) lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6 kDa band according to NP_003493.1 and 91.7 kDa band according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human adrenal gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Axin-1 Antibody

• axin 1
• Axin1
• Axin-1
• AXINaxis inhibitor 1
• Axis inhibition protein 1
• fused, mouse, homolog of
• hAxin
• MGC52315

Background

Negative regulator of Wnt signaling. Also associated with cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axin-1 Antibody (NB100-1011)(2)

Publications using NB100-1011

• Qadah T, Noorwali A, Alzahrani F et al. Detection of BCL11A and HBS1L-MYB Genotypes in Sickle Cell Anemia Indian Journal of Hematology & Blood Transfusion 2020-03-03 [PMID: 33100714]
• Zeng L, Fagotto F, Zhang T et al. The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation. Cell 1997-07-11 [PMID: 9230313]

Bioinformatics

• Gene Symbol: AXIN1
• Entrez:
  • 8312(Human)
  • 12005(Mouse)
  • 79257(Rat)
• Uniprot:
  • NP_003493(Human)