Axin-1 Antibody

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Immunohistochemistry: Axin-1 Antibody [NB100-1011] - (3.8ug/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Axin-1 Antibody Summary

Immunogen
Peptide with sequence C-FEEKIIGKVEKVD corresponding to C-Terminus according to NP_851393.1.
Specificity
This antibody is expected to recognise both reported isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AXIN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave bands at approx. 150 kDa band and 22-24 kDa band in human brain (hippocampus) lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6 kDa band according to NP_003493.1 and 91.7 kDa band according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human adrenal gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex.
Publications
Read Publication using NB100-1011.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Axin-1 Antibody

  • axin 1
  • Axin1
  • Axin-1
  • AXINaxis inhibitor 1
  • Axis inhibition protein 1
  • fused, mouse, homolog of
  • hAxin
  • MGC52315

Background

Negative regulator of Wnt signaling. Also associated with cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Axin-1 Antibody (NB100-1011)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AXIN1
Entrez
Uniprot