ATP6V0A2 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

ATP6V0A2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ATP6V0A2.
  
  

  Source: E. coli

  Amino Acid Sequence: EVKRCEELERILVYLVQEINRADIPLPEGEASPPAPPLKQVLEMQEQLQKLEVELREVTKNKEKLRKNLLELIEYTHMLRVTKTFVKRNVEFEPTYEEFPSLESDSLLDYSCMQRLGAKLGFVSGLINQGKVEAFE

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: ATP6V0A2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-91690.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for ATP6V0A2 Recombinant Protein Antigen

• A2
• ARCL
• ATP6A2
• ATP6N1D
• ATP6V0
• ATPase, H+ transporting, lysosomal V0 subunit a isoform 2
• ATPase, H+ transporting, lysosomal V0 subunit a2
• infantile malignant osteopetrosis
• J6B7
• Lysosomal H(+)-transporting ATPase V0 subunit a2
• regeneration and tolerance factor
• RTF
• STV1
• TJ6A2V-ATPase
• TJ6M
• TJ6s
• Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2
• vacuolar proton translocating ATPase 116 kDa subunit a
• V-ATPase 116 kDa isoform a2
• v-ATPase 116 kDa
• Vph1
• V-type proton ATPase 116 kDa subunit a isoform 2
• v-type proton ATPase 116 kDa subunit a
• WSS

Background

The multisubunit vacuolar-type proton pump (H(+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H(+)-ATPases are comprised of a peripheral V(1) domain and an integral membrane V(0) domain; ATP6V0A2 is a component of the V(0) domain (Smith et al., 2003 [PubMed 14580332]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ATP6V0A2