ATF7 Antibody (JJ2-072)

Immunocytochemistry/Immunofluorescence: ATF7 Antibody (JJ2-072) [NBP2-67857] - Staining ATF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ATF7 Antibody (JJ2-072) [NBP2-67857] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATF7 antibody. Counter stained with hematoxylin.

Flow Cytometry: ATF7 Antibody (JJ2-072) [NBP2-67857] - Analysis of Jurkat cells with ATF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: ATF7 Antibody (JJ2-072) [NBP2-67857] - Staining ATF7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ATF7 Antibody (JJ2-072) [NBP2-67857] - Analysis of paraffin-embedded mouse heart tissue using anti-ATF7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATF7 Antibody (JJ2-072) [NBP2-67857] - Analysis of paraffin-embedded mouse lung tissue using anti-ATF7 antibody. Counter stained with hematoxylin.

Product Discontinued

ATF7 Antibody (JJ2-072) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ATF7 aa 1-50 / 483. (SwissProt: P17544 Human; SwissProt: Q8R0S1 Mouse; Unigene: 219706 Rat)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATF7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ATF7 Antibody (JJ2-072)

• activating transcription factor 7ATFATranscription factor ATF-A
• cAMP-dependent transcription factor ATF-7
• cyclic AMP-dependent transcription factor ATF-7
• MGC57182

Background

The cyclic AMP-dependent transcription factor ATF7 is a member of the bZIP family of proteins and is known to bind to the cAMP response element (CRE) as a homodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF7