ATF6 Cell Lysate

Product Discontinued

ATF6 Cell Lysate Summary

• Description: 100 uls each of transfected and mock transfected human ATF6 cell lysate in 1X sample buffer.
• Gene: ATF6

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: HEK293 cells were transfected with full-length human ATF6. Load 20 ul/lane of a mini-gel. This transfected cell lysate can be used as a positive western blot control for NBP1-40256, anti-ATF6 mAb.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1 x SDS sample buffer: 60 mM Tris-Hcl (pH 6.8), 10 mM EDTA, 2% SDS, 0.03% Bromophenol Blue, 10% glycerol, and 0.3% beta-mercaptoethanol.

Lysate Details for Array

• Type: Cell

Alternate Names for ATF6 Cell Lysate

• ACHM7
• Activating transcription factor 6 alpha
• activating transcription factor 6
• atf6 a
• ATF6 alpha
• ATF6
• ATF6A
• ATF6-alpha
• cAMP-dependent transcription factor ATF-6 alpha
• cyclic AMP-dependent transcription factor ATF-6 alpha

Background

ATF6 is a constitutively expressed, endoplasmic reticulum (ER) membraneanchored transcription factor. ATF6 is a key transcriptional activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER (reviewed in Shen and Prywes, and Groenendyk and Michalak). The C-terminus of ATF6 is located in the ER lumen and its N-terminal DNA binding domain faces the cytosol. AFT6 plays a key role in the ER stress response by transmitting the ER stress signal across the ER membrane into the nucleus. The induction of new gene expression by ATF6 is an important aspect of the ER stress response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for ATF6 Lysate (NBP1-70778)(1)

Publication using NBP1-70778

• Roboti P, Swanton E, High S. Differences in endoplasmic-reticulum quality control determine the cellular response to disease-associated mutants of proteolipid protein. J Cell Sci. 2009-11-01 [PMID: 19825935] (WB)
  
  Details:
  Products cited (WB), Fig 5B (HeLa and HeLa overexpressing myelin proteolipid protein):1. ATF6 mAb (IMG-273).2. ATF6 Transfected and Mock Transfected Lysates (40210). The lysates were used as a positive control to validate the specificity of the antibody.

FAQs for ATF6 Lysate (NBP1-70778). (Showing 1 - 1 of 1 FAQ).

1. We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
   • The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.

Bioinformatics

• Gene Symbol: ATF6