ATF6 Cell Lysate

Images

 
Western Blot: ATF6 Cell Lysate [NBP1-70778] - 20 ul of human ATF6 A) transfected cell lysate and B) mock transfected cell lysate probed with 3 ug/ml of NBP1-70778.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ATF6 Cell Lysate Summary

Description
100 uls each of transfected and mock transfected human ATF6 cell lysate in 1X sample buffer.
Gene
ATF6

Applications/Dilutions

Application Notes
HEK293 cells were transfected with full-length human ATF6. Load 20 ul/lane of a mini-gel. This transfected cell lysate can be used as a positive western blot control for NBP1-40256, anti-ATF6 mAb.
Publications
Read Publication using NBP1-70778.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1 x SDS sample buffer: 60 mM Tris-Hcl (pH 6.8), 10 mM EDTA, 2% SDS, 0.03% Bromophenol Blue, 10% glycerol, and 0.3% beta-mercaptoethanol.

Lysate Details for ATF6

Type
Cell

Alternate Names for ATF6 Cell Lysate

  • Activating transcription factor 6 alpha
  • activating transcription factor 6
  • atf6 a
  • ATF6A
  • ATF6-alpha
  • cAMP-dependent transcription factor ATF-6 alpha
  • cyclic AMP-dependent transcription factor ATF-6 alpha

Background

ATF6 is a constitutively expressed, endoplasmic reticulum (ER) membraneanchored transcription factor. ATF6 is a key transcriptional activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER (reviewed in Shen and Prywes, and Groenendyk and Michalak). The C-terminus of ATF6 is located in the ER lumen and its N-terminal DNA binding domain faces the cytosol. AFT6 plays a key role in the ER stress response by transmitting the ER stress signal across the ER membrane into the nucleus. The induction of new gene expression by ATF6 is an important aspect of the ER stress response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ATF6 Lysate (NBP1-70778)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ATF6 Lysate (NBP1-70778). (Showing 1 - 1 of 1 FAQ).

  1. We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
    • The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.

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Bioinformatics Tool for ATF6 Lysate (NBP1-70778)

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Blogs on ATF6.

ATF6 - monitoring and regulating protein folding under cellular stress
During times of cellular stress overloading of the protein folding machinery leads to the accumulation of incorrectly folded proteins. This triggers the unfolded protein response (UPR) in order to try to reestablish homeostasis or, if this fails, t...  Read full blog post.

ATF6 - a key target in alcohol-induced fatty liver disease?
Untitled DocumentThe ATF6 endoplasmic reticulum (ER) stress-regulated transcription factor is constitutively expressed and plays a central role in the mammalian unfolded protein response (UPR). This fundamental pathway is responsibl...  Read full blog post.

A Key to Fight Stress: ATF6
The protein ATF6 is a constitutively expressed transcription factor that is a key mediator of the unfolded protein response (UPR) that allows mammalian cells to maintain cellular homeostasis under conditions of environmental and physiological stress....  Read full blog post.

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Bioinformatics

Gene Symbol ATF6