ATF6 Antibody

Product Discontinued

ATF6 Antibody Summary

• Immunogen: Synthetic peptide C-EATHVVSTIPESLQ, representing the C-Terminus of the human protein sequence according to NP_031374.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ATF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: This antibody can be used for ELISA and Western Blot. In Western Blot, Preliminary experiments gave an approx 25kDa band in HeLa lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 75kDa according to NP_031374. The 25 kDa band was successfully blocked by incubation with the immunizing peptide. Not tested in other applications. Recommended Starting Dilution:* ELISA (peptide): 1:16,000 WB: 1ug/ml *Optimal dilutions should be determined by the end user.

Reactivity Notes

Tested: Expected from sequence similarity: Human, Mouse

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for ATF6 Antibody

• ACHM7
• Activating transcription factor 6 alpha
• activating transcription factor 6
• atf6 a
• ATF6 alpha
• ATF6
• ATF6A
• ATF6-alpha
• cAMP-dependent transcription factor ATF-6 alpha
• cyclic AMP-dependent transcription factor ATF-6 alpha

Background

ATF6, a basic-leucine zipper protein, was isolated by yeast interaction assay as a binding partner to SRF (serum response factor) (1). SRF is a transcription factor, which binds to the serum response element (SRE) in the c-fos promoter and regulates expression of the c-fos gene as well as other immediate-early genes and some tissue-specific genes. ATF6 has also been shown to interact with the endoplasmic reticulum (ER) stress response element (ERSE) (2). ATF6 constitutively expressed as a membrane-bound 90-kDa protein is directly converted to a 50-kDa protein in ER-stressed cells (3). When unfolded proteins accumulate in the ER, ATF6 is released and translocates to the golgi where it is cleaved into its active form which enters the nucleus and activates translation of UPR genes including XBP1 (4).]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ATF6 Antibody (NB300-896). (Showing 1 - 1 of 1 FAQ).

1. We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
   • The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.

Bioinformatics

• Gene Symbol: ATF6
• Entrez:
  • 22926(Human)
• Uniprot:
  • NP_031374.(Human)