ATF2 [p Ser112, p Ser94] Antibody - BSA Free

Western Blot: ATF2 [p Ser112, p Ser94] Antibody [NBP2-54641] - Western blot analysis of lysed extracts from MDA-MB-435 cells using ATF2 [p Ser112, pSer94]

Immunohistochemistry-Paraffin: ATF2 [p Ser112, p Ser94] Antibody [NBP2-54641] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 [p Ser112, p Ser94]

Product Discontinued

ATF2 [p Ser112, p Ser94] Antibody - BSA Free Summary

• Immunogen: A peptide sequence around aa.110~114 or 92~96 (D-L-S-P-L) derived from Human ATF2
• Modification: p Ser112, p Ser94
• Specificity: This antibody detects endogenous level of total ATF2 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATF2
• Purity: Epitope affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000
• Theoretical MW: 65-75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Epitope affinity purified

Alternate Names for ATF2 [p Ser112, p Ser94] Antibody - BSA Free

• activating transcription factor 2formerly
• ATF2
• cAMP responsive element binding protein 2
• cAMP-responsive element-binding protein 2
• CREB2
• CREBP1
• CRE-BP1
• cyclic AMP-dependent transcription factor ATF-2
• HB16
• MGC111558
• mXBP
• TREB7

Background

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF2