Recombinant Human ASF1a His Protein

SDS-Page: Recombinant Human ASF1a His Protein [NBC1-22941] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1.0 mg/ml

Recombinant Human ASF1a His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-204 of Human ASF1a
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAKV QVNNVVVLDN PSPFYNPFQF EITFECIEDL SEDLEWKIIY VGSAESEEYD QVLDSVLVGP VPAGRHMFVF QADAPNPGLI PDADAVGVTV VLITCTYRGQ EFIRVGYYVN NEYTETELRE NPPVKPDFSK LQRNILASNP RVTRFHINWE DNTEKLEDAE SSNPNLQSLL STDALPSASK GWSTSENSLN VMLESHMDCM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ASF1A
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human ASF1a His Protein

• ASF1 silencing function 1 homolog A (S. cerevisiae)
• CCG1-interacting factor A
• CIACGI-98
• DKFZP547E2110
• hAsf1
• hAsf1a
• hCIA
• histone chaperone ASF1A
• HSPC146
• silencing function 1A
• silencing function protein 1 homolog A

Background

Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146 Description ASF1 anti-silencing function 1 homolog A, also known as ASF1A, belongs to H3/H4 family of histone chaperone proteins. ASF1A protein interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. Deletion of ASF1A in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint. Recombinant human ASF1A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ASF1A