ASF1a Antibody

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Western Blot: ASF1a Antibody [NB100-93376] - Analysis of ASF1a using this antibody at 0.01 ug/ml in Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ASF1a Antibody Summary

Immunogen
Peptide with sequence C-EDNTEKLEDAE corresponding to internal region according to NP_054753.1.
Epitope
C-EDNTEKLEDAE
Predicted Species
Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ASF1A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 23 kDa band observed in Jurkat lysates (calculated MW of 23.0 kDa band according to NP_054753.1).
Control
ASF1a Overexpression Lysate
Publications
Read Publication using NB100-93376.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASF1a Antibody

  • ASF1 silencing function 1 homolog A (S. cerevisiae)
  • CCG1-interacting factor A
  • CIACGI-98
  • DKFZP547E2110
  • hAsf1
  • hAsf1a
  • hCIA
  • histone chaperone ASF1A
  • HSPC146
  • silencing function 1A
  • silencing function protein 1 homolog A

Background

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASF1a Antibody (NB100-93376)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ASF1A
Entrez
Uniprot