ASF1a Antibody

Western Blot: ASF1a Antibody [NB100-93376] - Analysis of ASF1a using this antibody at 0.01 ug/ml in Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ASF1a Antibody Summary

• Description: Novus Biologicals Goat ASF1a Antibody (NB100-93376) is a polyclonal antibody validated for use in WB and ELISA. Anti-ASF1a Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EDNTEKLEDAE corresponding to internal region according to NP_054753.1.
• Epitope: C-EDNTEKLEDAE
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ASF1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 23 kDa band observed in Jurkat lysates (calculated MW of 23.0 kDa band according to NP_054753.1).
• Control:

  ASF1a Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ASF1a Antibody

• ASF1 silencing function 1 homolog A (S. cerevisiae)
• CCG1-interacting factor A
• CIACGI-98
• DKFZP547E2110
• hAsf1
• hAsf1a
• hCIA
• histone chaperone ASF1A
• HSPC146
• silencing function 1A
• silencing function protein 1 homolog A

Background

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASF1a Antibody (NB100-93376)(1)

Publication using NB100-93376

• Sanematsu F, Takami Y, Barman HK et al. Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells. J Biol Chem 2006-05-12 [PMID: 16537536]

Bioinformatics

• Gene Symbol: ASF1A
• Entrez:
  • 25842(Human)
  • 66403(Mouse)
  • 294408(Rat)
• Uniprot:
  • NP_054753.1(Human)