ASAH3 Overexpression Lysate

Images

 
Western Blot: ASAH3 Overexpression Lysate (Adult Normal) [NBL1-07748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASAH3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ASAH3 Overexpression Lysate Summary

Description
Transient overexpression lysate of alkaline ceramidase 1 (ACER1) The lysate was created in HEK293T cells, using Plasmid ID RC211332 and based on accession number NM_133492. The protein contains a C-MYC/DDK Tag.
Gene
ACER1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
30.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ASAH3 Overexpression Lysate

  • Acylsphingosine deacylase 3
  • Alkaline CDase 1
  • alkaline ceramidase 1
  • AlkCDase 1
  • ALKCDase1
  • ASAH3
  • EC 3.5.1.23
  • MGC138327
  • MGC138329
  • N-acylsphingosine amidohydrolase (alkaline ceramidase) 3
  • N-acylsphingosine amidohydrolase 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89296
Species: Hu
Applications: WB, IHC, IHC-P
NBP3-05160
Species: Hu
Applications: ICC/IF, IHC
NBP2-68894
Species: Hu
Applications: IHC, IHC-P
NBP1-83363
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2756
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-46290
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-33742
Species: Hu
Applications: Simple Western, ICC/IF, IHC, IHC-P
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-69285
Species: Hu
Applications: WB
NBP1-82648
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-59941
Species: Hu
Applications: WB, IHC, IHC-P

Publications for ASAH3 Lysate (NBL1-07748) (0)

There are no publications for ASAH3 Lysate (NBL1-07748).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ASAH3 Lysate (NBL1-07748) (0)

There are no reviews for ASAH3 Lysate (NBL1-07748). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ASAH3 Lysate (NBL1-07748) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-07748

Additional ASAH3 Products

Array NBL1-07748

Bioinformatics Tool for ASAH3 Lysate (NBL1-07748)

Discover related pathways, diseases and genes to ASAH3 Lysate (NBL1-07748). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ASAH3 Lysate (NBL1-07748)

Discover more about diseases related to ASAH3 Lysate (NBL1-07748).
 

Pathways for ASAH3 Lysate (NBL1-07748)

View related products by pathway.

PTMs for ASAH3 Lysate (NBL1-07748)

Learn more about PTMs related to ASAH3 Lysate (NBL1-07748).

Blogs on ASAH3

There are no specific blogs for ASAH3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ASAH3 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ACER1