ASAH3 Overexpression Lysate Summary
| Description |
Transient overexpression lysate of alkaline ceramidase 1 (ACER1) The lysate was created in HEK293T cells, using Plasmid ID RC211332 and based on accession number NM_133492. The protein contains a C-MYC/DDK Tag. |
| Gene |
ACER1 |
Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot.
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). |
| Theoretical MW |
30.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
RIPA buffer |
Lysate Details for Array
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for ASAH3 Overexpression Lysate
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Blogs on ASAH3
![Western Blot: ASAH3 Overexpression Lysate (Adult Normal) [NBL1-07748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASAH3.](http://images.novusbio.com/fullsize/ASAH3 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-07748-img0002.jpg "Western Blot: ASAH3 Overexpression Lysate (Adult Normal) [NBL1-07748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASAH3.")
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![Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/ASAH1-Antibody-Immunohistochemistry-Paraffin-NBP1-89296-img0013.jpg)
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![Immunocytochemistry/Immunofluorescence: MBTPS1 Antibody [NBP3-05160] - Analysis of U2OS cells using MBTPS1 antibody. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/MBTPS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05160-img0001.jpg)
![Immunohistochemistry-Paraffin: MBTPS1 Antibody [NBP3-05160] - Paraffin-embedded human stomach using MBTPS1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/images2/MBTPS1 Antibody-Immunohistochemistry-Paraffin-NBP3-05160-img0004.jpg)
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![Western Blot: PSAP Antibody [NBP1-83363] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/images2/PSAP-Antibody-Western-Blot-NBP1-83363-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PSAP Antibody [NBP1-83363] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/PSAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83363-img0016.jpg)
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