| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clone | OTI6E2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | HRP |
| Immunogen | Recombinant protein fragment corresponding to amino acids 83-295 of human ART1 (NP_004305) produced in E.coli. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ART1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Immunogen affinity purified |
![ART1 Antibody (OTI6E2) [HRP]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "ART1 Antibody (OTI6E2) [HRP]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-70211AF350 | |
| Alexa Fluor 405 | NBP2-70211AF405 | |
| Alexa Fluor 488 | NBP2-70211AF488 | |
| Alexa Fluor 532 | NBP2-70211AF532 | |
| Alexa Fluor 594 | NBP2-70211AF594 | |
| Alexa Fluor 647 | NBP2-70211AF647 | |
| Alexa Fluor 700 | NBP2-70211AF700 | |
| Alexa Fluor 750 | NBP2-70211AF750 | |
| Biotin | NBP2-70211B | |
| DyLight 350 | NBP2-70211UV | |
| DyLight 405 | NBP2-70211V | |
| DyLight 488 | NBP2-70211G | |
| DyLight 550 | NBP2-70211R | |
| DyLight 594 | NBP2-70211DL594 | |
| DyLight 650 | NBP2-70211C | |
| DyLight 680 | NBP2-70211FR | |
| DyLight 755 | NBP2-70211IR | |
| HRP | NBP2-70211H |
Diseases for ART1 Antibody (NBP2-70211H)Discover more about diseases related to ART1 Antibody (NBP2-70211H).
| Pathways for ART1 Antibody (NBP2-70211H)View related products by pathway.
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PTMs for ART1 Antibody (NBP2-70211H)Learn more about PTMs related to ART1 Antibody (NBP2-70211H).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ART1 |
![Flow Cytometry: ART2.2/Art2b Antibody (Nika102) [NB100-65853] - Staining of C57Bl/6J mouse spleen cells with RAT ANTI MOUSE ART2.2 antibody (NB100-65853).](http://images.novusbio.com/images/ART2.2-Art2b Antibody (Nika102)-Flow Cytometry-NB100-65853-img0001.jpg)
![Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated at 4C overnight.](http://images.novusbio.com/images2/P2X7-P2RX7-Antibody-Western-Blot-NBP1-20180-img0006.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen.Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/images2/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0022.jpg)
![Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - Rat brain lysate using Rabbit antibody to P2RX7 at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20](http://images.novusbio.com/images2/P2X7-P2RX7-Antibody-Western-Blot-NBP1-20180-img0005.jpg)
![Immunohistochemistry-Paraffin: ART3 Antibody (3A2) [H00000419-M05] - Analysis of monoclonal antibody to ART3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 2 ug/ml](http://images.novusbio.com/images2/ART3-Antibody-(3A2)-Immunohistochemistry-Paraffin-H00000419-M05-img0003.jpg)
![ELISA: ART3 Antibody (3A2) [H00000419-M05] - Detection limit for recombinant GST tagged ART3 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/images2/ART3-Antibody-(3A2)-ELISA-H00000419-M05-img0002.jpg)
![Western Blot: GNPDA1 Antibody [NBP2-24653] - analysis of GNPDA1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen and 4) rat spleen using GNPDA1 antibody at 1.0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.](http://images.novusbio.com/images2/GNPDA1 Antibody-Western Blot-NBP2-24653-img0001.jpg)
![Immunohistochemistry-Paraffin: GNPDA1 Antibody [NBP2-24653] - Formalin-fixed paraffin-embedded human kidney tissue stained with GNPDA1 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/images2/GNPDA1-Antibody-Immunohistochemistry-Paraffin-NBP2-24653-img0002.jpg)
![Western Blot: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] Analysis of extracts (35ug) from 9 different cell lines by using anti-Glucose 6 phosphate isomerasemonoclonal antibody.](http://images.novusbio.com/images/Glucose 6 phosphate isomerase Antibody (2D2)-Western Blot-NBP2-02541-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glucose 6 phosphate isomerase.](http://images.novusbio.com/images/Glucose 6 phosphate isomerase Antibody (2D2)-Immunocytochemistry-Immunofluorescence-NBP2-02541-img0007.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Human prostate tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.](http://images.novusbio.com/images/Glucose 6 phosphate isomerase Antibody (2D2)-Immunohistochemistry-Paraffin-NBP2-02541-img0006.jpg)
![Western Blot: ART4 Antibody [NBP1-80507] - Sample Tissue: Human Stomach Tumor Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/images2/ART4-Antibody-Western-Blot-NBP1-80507-img0003.jpg)
![Western Blot: ART4 Antibody [NBP1-80507] - Human Heart lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/images2/ART4-Antibody-Western-Blot-NBP1-80507-img0002.jpg)
![Western Blot: ART5 Antibody [NBP1-32664] - Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/images2/ART5 Antibody-Western Blot-NBP1-32664-img0004.jpg)
![Immunohistochemistry-Paraffin: ART5 Antibody [NBP1-32664] - Mouse prostate. ART5 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/images2/ART5-Antibody-Immunohistochemistry-Paraffin-NBP1-32664-img0005.jpg)
![Immunohistochemistry-Paraffin: ART5 Antibody [NBP1-32664] - SAS xenograft, using ART5 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/images2/ART5 Antibody-Immunohistochemistry-Paraffin-NBP1-32664-img0003.jpg)
![Immunohistochemistry-Paraffin: NOB1 Antibody [NBP2-13663] - Staining of human ovary shows nuclear positivity in ovarian stroma cells.](http://images.novusbio.com/images2/NOB1 Antibody-Immunohistochemistry-Paraffin-NBP2-13663-img0003.jpg)
![Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis using Azide Free version of NBP2-25250. Lane view shows a specific band for CD38 in 0.2 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/CD38-Antibody-(1G7F4)-Simple-Western-NBP2-25250-img0011.jpg)
![Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - B cell lymphoma tumor sections (Ramos). Antibody Dilution 1:200. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/images2/CD38-Antibody-(1G7F4)-Immunohistochemistry-Frozen-NBP2-25250-img0012.jpg)
![Western Blot: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis of (A) Human Jurkat, (B) Human Ramos and (C) Mouse EL4 lysate using this antibody at 4 ug/mL.](http://images.novusbio.com/images2/CD38-Antibody-(1G7F4)-Western-Blot-NBP2-25250-img0003.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ATN1 Antibody [NBP1-90044] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/ATN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90044-img0012.jpg)
![Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/ATN1-Antibody-Immunohistochemistry-Paraffin-NBP1-90044-img0016.jpg)
![Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Analysis in human cerebral cortex and liver tissues. Corresponding ATN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/ATN1-Antibody-Immunohistochemistry-Paraffin-NBP1-90044-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0011.jpg)
![Immunohistochemistry: CD8 Antibody (53-6.7) [NBP1-49045] - Histopathological analysis of spinal cord in EAE mice. Representative spinal cord sections from wild-type (WT), Asic1-/-, and Asic2-/- mice at 45 days after immunization. Sections of the spinal cord anterior horn were stained with hematoxylin and eosin (E&H) to assess inflammation and immunostained for MCHII, CD4+, CD8+ infiltrating cells. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1111/ejn.14302), licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-NBP1-49045-img0016.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Flow-Cytometry-NBP1-49045-img0014.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/images2/PARP-Antibody-(194C1439)---Cleaved-Western-Blot-NB100-56599-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/PARP-Antibody-(194C1439)---Cleaved-Immunocytochemistry-Immunofluorescence-NB100-56599-img0005.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.](http://images.novusbio.com/images2/PARP-Antibody-(194C1439)---Cleaved-Western-Blot-NB100-56599-img0003.jpg)