Recombinant Human ARL5A His Protein

SDS-Page: Recombinant Human ARL5A Protein [NBP1-50900] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human ARL5A His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-179 of Human ARL5A
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGILFT RIWRLFNHQE HKVIIVGLDN AGKTTILYQF SMNEVVHTSP TIGSNVEEIV INNTRFLMWD IGGQESLRSS WNTYYTNTEF VIVVVDSTDR ERISVTREEL YKMLAHEDLR KAGLLIFANK QDVKECMTVA EISQFLKLTS IKDHQWHIQA CCALTGEGLC QGLEWMMSRL KIR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ARL5A
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Use in dot blot reported in scientific literature (PMID 26028028)
• Theoretical MW: 23.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Analogous biological activity in mouse tissues/cells reported in scientific literature (PMID: 26028028)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 0.2 mM PMSF
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human ARL5A His Protein

• ADP-ribosylation factor-like 5
• ADP-ribosylation factor-like 5A
• ADP-ribosylation factor-like protein 5A
• ARFLP5
• ARL5ADP-ribosylation factor-like protein 5

Background

ADP-ribosylation factor-like 5A, also known ARL5A, belongs to the ARF family of GTP-binding proteins. ARFs are important in eukaryotic vesicular trafficking pathways and play an essential role in the activation of phospholipase D. Unlike many ARF family members, ARL5 is thought to lack ADP-ribosylation enhancing activity. ARL5A is developmentally regulated and may play a role in nuclear dynamics and signaling cascades during embryonic development. Recombinant human ARL5A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for ARL5A Recombinant Protein (NBP1-50900)(1)

Publication using NBP1-50900

• Ho JC, Nadeem A, Rydstrom A et al. Targeting of nucleotide-binding proteins by HAMLET-a conserved tumor cell death mechanism Oncogene. 2015-06-01 [PMID: 26028028] (Cytometric Bead Assay Standard, Human)

Bioinformatics

• Gene Symbol: ARL5A