Argonaute 3 Overexpression Lysate

Images

 
Western Blot: Argonaute 3 Overexpression Lysate (Adult Normal) [NBL1-10184] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Argonaute 3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Argonaute 3 Overexpression Lysate Summary

Description
Transient overexpression lysate of eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC222233 and based on accession number NM_024852. The protein contains a C-MYC/DDK Tag.
Gene
AGO3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
97.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Argonaute 3 Overexpression Lysate

  • AGO3argonaute 3
  • argonaute3
  • eIF2C 3
  • eIF-2C 3
  • Eukaryotic translation initiation factor 2C 3
  • eukaryotic translation initiation factor 2C, 3
  • FLJ12765
  • hAgo3
  • MGC86946
  • protein argonaute-3

Background

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-10184

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Bioinformatics

Gene Symbol AGO3